sw1353  (DSMZ)

Journal: International journal of molecular sciences

Article Title: Targeting Bone Tumours with 45S5 Bioactive Glass.

doi: 10.3390/ijms251910830

Figure Lengend Snippet: Figure 7. 45S5-BG inhibits tumour formation in vivo. OS, GCTB and CS cells were co-transplanted with 45S5-BG at the indicated concentrations onto the CAM of fertilised chicken eggs (n = 20 for each experimental group). Tumours that formed after seven days were resected, and (A) the tumour take rate, (B) the volume of the individual tumours (white rectangles indicate the mean tumour volumes), and (C) the cumulative tumour volume of the experimental groups were calculated. (D) Photographs of the resected tumours. Representative data from the OS cell line MNNG-HOS, the CS cell line SW1353, and a primary GCTB cell line are shown.

Article Snippet: The following five commercially available human osteosarcoma cell lines and two chondrosarcoma cell lines were used in this study: HOS 143B (Sigma-Aldrich, Taufkirchen, Germany), CAL-72 (DSMZ, Braunschweig, Germany), MNNG-HOS, Saos-2, and U2OS (Cell Line Service GmbH, Eppelheim, Germany), SW1353 (Cell Line Service GmbH), and CAL-78 (DSMZ).

Techniques: In Vivo

Journal: International Journal of Molecular Sciences

Article Title: DNA-PKcs Inhibition Sensitizes Human Chondrosarcoma Cells to Carbon Ion Irradiation via Cell Cycle Arrest and Telomere Capping Disruption

doi: 10.3390/ijms25116179

Figure Lengend Snippet: ( A ) Chemical structure of 7,9-dihydro-7-methyl-2-[(7-methyl[1,2,4]triazolo[1,5-a]pyridin-6-yl)amino]-9-(tetrahydro-2H-pyran-4-yl)-8H-purin-8-one (AZD7648). ( B ) The dose–response relationship after treatment with 0.03 and 100 µM AZD7648 with and without IR. The relative gene expression of the proto-oncogene cMyc, cyclin D1, and survivin, which play a role in cell cycle progression, apoptosis, and cellular transformation. The SW-1353 (dark grey) and Cal78 (light grey) chondrosarcoma cells were treated with 3 µM AZD7648 alone, respectively, in a combined treatment with ( C ) 8 Gy X-ray or ( D ) 8 Gy C-ion IR (mean ± SD, n = 6, measured in triplicates). Statistical significances to the untreated controls (ctrl 0 Gy) are defined as follows: * p < 0.05; ** p < 0.01; *** p < 0.001. Statistical significances between the 3 µM AZD7648 group and the combined treatment with IR are presented as # p < 0.05; ## p < 0.01; ### p < 0.001.

Article Snippet: The SW-1353 (ATCC ® HTB-94TM, LGC Standards, Middlesex, UK) and Cal78 (ACC449; DSMZ, Leibniz, Germany) chondrosarcoma cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-HG) supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and 0.25 μg amphotericin B (all GIBCO ® , Invitrogen, Darmstadt, Germany).

Techniques: Gene Expression, Transformation Assay

Journal: International Journal of Molecular Sciences

Article Title: DNA-PKcs Inhibition Sensitizes Human Chondrosarcoma Cells to Carbon Ion Irradiation via Cell Cycle Arrest and Telomere Capping Disruption

doi: 10.3390/ijms25116179

Figure Lengend Snippet: Cell cycle distribution of the chondrosarcoma cell lines 48 h after the combined treatment of 3 µM AZD7648 and 8 Gy X-ray, respectively, and C-ion IR (n = 5; mean ± SD; n.s.: not significant). Statistical significances to the non-irradiated controls (ctrl 0 Gy) are defined as follows: * p < 0.05; ** p < 0.01; *** p < 0.001. Statistical significances between irradiated controls (X-ray 8 Gy, respectively, C-ions 8 Gy) to the combined treatment with 3 µM AZD7648 are presented as # p < 0.05; ## p < 0.01; ### p < 0.001.

Article Snippet: The SW-1353 (ATCC ® HTB-94TM, LGC Standards, Middlesex, UK) and Cal78 (ACC449; DSMZ, Leibniz, Germany) chondrosarcoma cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-HG) supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and 0.25 μg amphotericin B (all GIBCO ® , Invitrogen, Darmstadt, Germany).

Techniques: Irradiation

Journal: International Journal of Molecular Sciences

Article Title: DNA-PKcs Inhibition Sensitizes Human Chondrosarcoma Cells to Carbon Ion Irradiation via Cell Cycle Arrest and Telomere Capping Disruption

doi: 10.3390/ijms25116179

Figure Lengend Snippet: Cell cycle distribution after the combined treatment of the DNA-PKcs inhibitor AZD7648 and 8 Gy X-ray or C-ion IR. ( A ) The SW-1353 and ( C ) Cal78 chondrosarcoma cells were analyzed using flow cytometry 24 h after the combined treatment with 3 µM AZD7648 and 8 Gy X-ray or C-ion IR (LET ca. 55 keV/μm). The statistical evaluation is presented in stacked bar charts (n = 5). The distribution of cells in the G 1 /G 0 phase (black), the S-phase (light gray), and the G 2 /M phase (dark gray) are shown in % of gated cells. ( B , D ) The representative original tracks of non-IR control cells (ctrl 0 Gy), 3 µM AZD7648 0 Gy (AZD 3 µM 0 Gy), and after 8 Gy X-ray, respectively, and C-ion IR with and without the AZD7648 treatment are shown. In both cell lines, C-ion IR induced a stronger G 2 /M arrest and a synergistic effect of the combined treatments.

Article Snippet: The SW-1353 (ATCC ® HTB-94TM, LGC Standards, Middlesex, UK) and Cal78 (ACC449; DSMZ, Leibniz, Germany) chondrosarcoma cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-HG) supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and 0.25 μg amphotericin B (all GIBCO ® , Invitrogen, Darmstadt, Germany).

Techniques: Flow Cytometry, Control

Journal: International Journal of Molecular Sciences

Article Title: DNA-PKcs Inhibition Sensitizes Human Chondrosarcoma Cells to Carbon Ion Irradiation via Cell Cycle Arrest and Telomere Capping Disruption

doi: 10.3390/ijms25116179

Figure Lengend Snippet: G 2 /M checkpoint genes regulation. The protein expression of the cell cycle G2/M phase key regulators cyclin B, cyclin-dependent kinase (CDK)1, and p53 was analyzed by immunoblotting in two chondrosarcoma cell lines under control conditions (ctrl 0 Gy), irradiated with ( A ) 8 Gy X-ray or ( B ) 8 Gy C-ion IR (ctrl 8 Gy), and after the combined treatment with 1 µM, 3 µM, and 10 µM AZD7648. β-actin was used as loading control (mean ± SD of n = 3). The full-length blots are presented in the . The AZD7648 treatment sustainably reduced the expression of these cell cycle regulators, both 24 h and 72 h after IR. ( B ) The relative gene expression of the G 2 /M checkpoint genes cyclin B, CDK1, and WEE1 was significantly reduced by IR alone and the combined treatment. The SW-1353 (dark grey) and Cal78 (light grey) chondrosarcoma cells were treated with 3 µM AZD7648 alone, respectively, in a combined treatment with ( C ) 8 Gy X-ray or ( D ) 8 Gy C-ion IR (mean ± SD, n = 6, measured in triplicates). Statistical significances to the untreated controls (ctrl 0 Gy) are defined as follows: * p < 0.05; ** p < 0.01; *** p < 0.001. Statistical significances between the 3 µM AZD7648 group and the combined treatment with IR are presented as # p < 0.05; ## p < 0.01; ### p < 0.001.

Article Snippet: The SW-1353 (ATCC ® HTB-94TM, LGC Standards, Middlesex, UK) and Cal78 (ACC449; DSMZ, Leibniz, Germany) chondrosarcoma cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-HG) supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and 0.25 μg amphotericin B (all GIBCO ® , Invitrogen, Darmstadt, Germany).

Techniques: Expressing, Western Blot, Control, Irradiation, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: DNA-PKcs Inhibition Sensitizes Human Chondrosarcoma Cells to Carbon Ion Irradiation via Cell Cycle Arrest and Telomere Capping Disruption

doi: 10.3390/ijms25116179

Figure Lengend Snippet: MAPK phosphorylation and the expression of protein stability markers. The p-AKT and p-Chk2 protein phosphorylation pattern of the SW-1353 and Cal78 chondrosarcoma cells 1 h after the combined treatment with 1 µM, 3 µM, or 10 µM AZD7648 and ( A ) 8 Gy X-ray, respectively ( B ) C-ion IR. While AKT phosphorylation was significantly downregulated, Chk2 phosphorylation was increased by the additional AZD7648 treatment. β-actin was used as loading control (mean ± SD of n = 3). The full-length blots are presented in the . The relative gene expression of the protein stability marker HSP90 and ( D ) AMPK 24 h after combined treatment with 3 µM AZD7648 and ( C ) 8 Gy X-ray, respectively, ( D ) 8 Gy C-ion IR (SW-1353: dark grey; Cal78: light grey) (mean ± SD; n = 6; measured in triplicates). Non-IR cells (ctrl 0 Gy) were used as controls (ratio = 1). Statistical significances to the untreated controls (ctrl 0 Gy) are defined as follows: * p < 0.05; ** p < 0.01; *** p < 0.001. Statistical significances between the 3 µM AZD7648 group and the combined treatment with IR are presented as # p < 0.05; ## p < 0.01; ### p < 0.001.

Article Snippet: The SW-1353 (ATCC ® HTB-94TM, LGC Standards, Middlesex, UK) and Cal78 (ACC449; DSMZ, Leibniz, Germany) chondrosarcoma cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-HG) supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and 0.25 μg amphotericin B (all GIBCO ® , Invitrogen, Darmstadt, Germany).

Techniques: Phospho-proteomics, Expressing, Control, Gene Expression, Marker

Journal: International Journal of Molecular Sciences

Article Title: DNA-PKcs Inhibition Sensitizes Human Chondrosarcoma Cells to Carbon Ion Irradiation via Cell Cycle Arrest and Telomere Capping Disruption

doi: 10.3390/ijms25116179

Figure Lengend Snippet: Relative gene expression of the DNA repair marker XRCC4 and die MDM2-p53 feedback loop. The SW-1353 (dark grey) and Cal78 (light grey) chondrosarcoma cells were treated with 3 µM AZD7648 alone, respectively, in a combined treatment with ( A ) 8 Gy X-ray or ( B ) 8 Gy C-ion IR (mean ± SD, n = 6, measured in triplicates). Statistical significances to the untreated controls are defined as follows: * p < 0.05; ** p < 0.01; *** p < 0.001. Statistical significances between the 3 µM AZD7648 group and the combined treatment with IR are presented as # p < 0.05; ## p < 0.01; ### p < 0.001.

Article Snippet: The SW-1353 (ATCC ® HTB-94TM, LGC Standards, Middlesex, UK) and Cal78 (ACC449; DSMZ, Leibniz, Germany) chondrosarcoma cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-HG) supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and 0.25 μg amphotericin B (all GIBCO ® , Invitrogen, Darmstadt, Germany).

Techniques: Gene Expression, Marker

Journal: International Journal of Molecular Sciences

Article Title: DNA-PKcs Inhibition Sensitizes Human Chondrosarcoma Cells to Carbon Ion Irradiation via Cell Cycle Arrest and Telomere Capping Disruption

doi: 10.3390/ijms25116179

Figure Lengend Snippet: Telomere length reduction in the chondrosarcoma cells after combined treatment with AZD7648 and 8 Gy X-ray/C-ion IR. The SW-1353 and Cal78 cells were incubated with 3 µM AZD7648 (dashed light grey bars) and irradiated with ( A ) either 8 Gy X-ray or ( B ) 8 Gy C-ion IR (dark grey bars). After 3, 10, and 30 days, the DNA was isolated and the telomere length was determined. Statistical significances between the control group (ctrl 0 Gy) and the treated groups are defined as follows: * p < 0.05; ** p < 0.01; *** p < 0.001. Statistical significances between the 3 µM AZD7648 group (dashed light grey bars) and the combined treatment with IR (dashed dark grey bars) are presented as # p < 0.05; ## p < 0.01; ### p < 0.001.

Article Snippet: The SW-1353 (ATCC ® HTB-94TM, LGC Standards, Middlesex, UK) and Cal78 (ACC449; DSMZ, Leibniz, Germany) chondrosarcoma cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM-HG) supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, and 0.25 μg amphotericin B (all GIBCO ® , Invitrogen, Darmstadt, Germany).

Techniques: Incubation, Irradiation, Isolation, Control